Effects of progesterone on the expressions of nerve growth factor and brain-derived neurotrophic factor after focal cerebral ischemia-reperfusion injury in rats
10.3760/cma.j.issn.0254-9026.2012.07.022
- VernacularTitle:黄体酮对大鼠局灶性脑缺血再灌注损伤后脑组织神经生长因子表达的影响
- Author:
Xin LI
;
Jianping WANG
;
Hong LU
;
Chao JIANG
;
Chunling LIU
- Publication Type:Journal Article
- Keywords:
Progesterone;
Reperfusion injury;
Nerve growth factors;
Brain ischemia
- From:
Chinese Journal of Geriatrics
2012;31(7):615-618
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of progesterone on the mRNA and protein expressions of nerve growth factor(NGF) and brain-derived neurotrophic factor(BDNF) in the focal cerebral ischemia-reperfused rats,and to explore its mechanism of brain protection. Methods Totally 96 healthy male SD rats were randomly divided into sham surgery group,ischemic reperfusion group,vehicle-treated group and progesterone-treated group (n=24 for each).The model of focal brain ischemia-reperfusion injury was induced by middle cerebral artery occlusion (MCAO).After 2 h temporary MCAO,rats were subjected to reperfusion for 3 h,6 h,12 h and 24 h,respectively.The mRNA and protein expressions of NGF and BDNF were analyzed by real time-PCR and Western blot,respectively. Results In the injured cortex,the mRNA and protein expressions of NGF and BDNF in ischemic reperfusion group came to the peak at 6 h after reperfusion,and then gradually declined to the level of sham operation group at 24 h after reperfusion.In progesterone treatment group,BDNF and NGF mRNA and protein expressions reach the peak at 12 h after reperfusion,and were still higher at 24 h after reperfusion than in ischemic reperfusion group(P＜0.05). Conclusions Progesterone plays a protective role in focal cerebral ischemia-reperfusion by increasing the expressions of BDNF and NGF in rats.
