Effects of oldhamianoside Ⅱ on the invasive ability of prostatic carcinoma cells
10.3760/cma.j.issn.1673-422X.2012.07.022
- VernacularTitle:霞草苷Ⅱ对前列腺癌细胞侵袭力的影响
- Author:
Yan WANG
;
Fengling WANG
;
Yanling MU
;
Jun LI
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
Matrix metalloproteinases;
Receptors;
CXCR4;
Oldhamianoside Ⅱ
- From:
Journal of International Oncology
2012;39(7):551-554
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of oldhamianoside Ⅱ on the invasive 'ability of prostatic carcinoma DU-145 cells,and to investigate the potential mechanism.Methods After treatment of DU-145 cells with oldhamianoside Ⅱ,MTT assay was used to test the effect of oldhamianoside Ⅱ on the cell proliferation.The invasive ability was assessed with a transwell cell culture chamber.Immunocytochemistry stain was used to investigate the expression of matrix metalloproteinase(MMP-2)and CXC-chemokine receptor 4 (CXCR4).Results The proliferation of DU-145 cells was inhibited after treatment with oldhamianoside Ⅱ.In vitro invasion assay indicated that the cells moved through the membrane were(180.3 ± 14.6)/field in the control group,while decreased to(100.4 ±1.5),(80.2±2.7)and(60.1 ±5.3)/field in 0.25,0.5 and 1μg/mL oldhamianoside Ⅱ treated DU-145 cells respectively.Comparing to control group,the cells of oldhamianoside Ⅱ groups moved through the membrane were decreased remarkably(P ＜0.05).Immunocytochemistry showed that the expression of MMP-2 and CXCR4 were decreased of oldhamianoside Ⅱ groups.Conclusions Oldhamianoside Ⅱ can inhibit the invasiveness of DU-145 cells,which is related to the down regulation the expression of MMP-2 in DU-t45 cells.Oldhamianoside Ⅱ can inhibit the prostate cancer cells metastasis to the bone,which is related to the down regulation the expression of CXCR4 in DU-145 cells.
