Effects of AKT2 expression on gemcitabine sensitivity in pancreatic cancer cell line Panc-1

VernacularTitle:胰腺癌细胞Panc-1中AKT2基因表达对吉西他滨敏感性的影响
Author: Tao PENG ; Feng ZHOU ; Peng YANG ; Chunyou WANG
Publication Type:Journal Article
Keywords: Pancreatic cancer; Gene; Gemcitabine
From: Chinese Journal of Hepatobiliary Surgery 2012;18(6):459-461
CountryChina
Language:Chinese
Abstract: Objective To explore the effects of AKT2 expression in pancreatic cancer cell line Panc-1 on the sensitivity towards to gemcitabine.Methods The expression vector pAKT2 shRNA was constructed and transfected into Panc-1 cells by lipofecton.The mRNA and protein expression levels of AKT2 were detected by RT-PCR and Western blot assays,respectively.The changes of gemcitabine sensitivity after shRNA were examined by the MTT assay.Results The mRNA and protein levels of AKT2 in Panc-1 cells were significantly decreased after the transfection,and the median inhibition concentration of gemcitabine against Panc-1 cells was reduced from (1.96 ± 0.22) mg/L to (0.24±0.03) mg/L.The sensitivity of Panc-1 cells to gemcitabine was increased significantly after pAKT2-shRNA transfection.Conclusion The sensitivity of Panc-1 cells to gemcitabine could be enhanced by pAKT2-shRNA.