Changes in expression of acid-sensing ion channel 3 in dorsal root ganglion in a rat model of bone cancer
10.3760/cma.j.issn.0254-1416.2012.05.013
- VernacularTitle:骨癌痛大鼠背根神经节酸感受离子通道3表达的变化
- Author:
Fang QIU
;
Weixiu YUAN
;
Weidong MI
;
Xiaoli WEI
;
Yanhong LIU
- Publication Type:Journal Article
- Keywords:
Bone neoplasms;
Pain;
lon channels;
Hydrogen-ion concentration;
Ganglia,spinal
- From:
Chinese Journal of Anesthesiology
2012;32(5):566-568
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in the expression of acid-sensing ion channel 3(ASIC3)in the dorsal root ganglion(DRG)in a rat model of bone cancer pain.Methods Twenty-four female SD rats,aged 3-4 yr,weighing 180-220 g,were randomly divided into 2 groups:sham operation group(group S,n =8)and bone cancer pain group(group P,n =16).Bone cancer pain was induced by intra-tibial inoculation of 10 μl Walker 256 cancer cell suspension in group P,while group S received intra-tibial inoculation of 5 μl normal saline.Body weight and paw withdrawal threshold to mechanical stimulation with yon Frey filaments(MWT)were measured at 0,1,3,5,7,9,1 1 and 14 d after cancer cell inoculation.The tibia was removed at 14 d after cancer cell inoculation in group S and at 7 and 14 d after cancer cell inoculation in group P for pathological and imaging examinations.The tumor cell growth and bony destruction were observed.The expression of ASIC3 in the DRG was determined by immunolluorescence.Results Pathological damage occurred at 14 d after cancer cell inoculation,bony destruction was observed obviously,ant cortical bone was missing in many places.Compared with group S,body weight at T3-7 and MWT al T2-7:were significantly decreaed,and the expression of ASIC3 was up-regulated at 14 d after cancer cell inoculation in group P(P ＜ 0.05).Conclusion Up-regulation of the expression of ASIC3 in the DRG is involved in the developntent and maiutenence ot bone cancer pain in rars.
