The expressions of endothelin-1 and endothelin A/B receptors mRNA in tissue of benign prostatic hyperplasia treated by daily low-dose sildenafil
10.3760/cma.j.issn.0254-9026.2012.06.017
- VernacularTitle:持续小剂量西地那非对良性前列腺增生患者组织内皮素-1及其受体的影响
- Author:
Hongde CHEN
;
Xueting YE
;
Yirong ZHANG
;
Zhiliang WENG
;
Chendi LI
- Publication Type:Journal Article
- Keywords:
Phosphodiesterase inhibitors;
Prostatic hyperplasia;
Receptor,endothelin A;
Receptor,endothelin B
- From:
Chinese Journal of Geriatrics
2012;31(6):500-502
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the mRNA expressions of endothelin-1(ET-1) and endothelin A/B receptors (ETA/B) in tissue of benign prostatic hyperplasia treated by daily low-dose sildenafil.Methods A total of 32 patients with benign prostatic hyperplasia were randomly divided into two groups:treatment(25 mg sildenafi for 12 weeks,n=16) and control (no drug,n=16) groups.Immunohistochemical staining,ELISA and RT-PCR were used to detect the expression levels of ET-1 protein and ET A/B mRNA,respectively.Results The expressions of ET-1 protein and ET A/B mRNA in prostatic tissue were significantly lower in treatment group than in control group[(53.31±18.56) ng/kg vs.(83.34±31.38) ng/kg,0.356±0.056 vs.0.624±0.083,0.721±0.083 vs.0.933±0.905,t=-3.295,10.715,6.937,all P＜0.001].Conclusions Daily low-dose sildenafil can reduce the expressions of ET-1 and ET A/B receptors mRNA in benign prostatic hyperplasia.
