Effects of penehyclidine hydrochloric pretreatment on expression of β-arrestin-2 in the lung tissue in sepsisInduced acute lung injury in mice
10.3760/cma.j.issn.0254-1416.2012.05.021
- VernacularTitle:盐酸戊乙奎醚预先给药对脓毒症小鼠急性肺损伤时肺组织β-arrestin-2表达的影响
- Author:
Jinjie LI
;
Fei XIAO
;
Jia ZHAN
;
Zongze ZHANG
;
Kai CHEN
;
Yanlin WANG
;
Yi HAN
- Publication Type:Journal Article
- Keywords:
Cholinergic antagonisls;
Arrestin;
Lung;
Sepsis;
Respiratory distress syndrome,adult
- From:
Chinese Journal of Anesthesiology
2012;32(5):593-596
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of penehyclidine hydrochloric(PHC)pretreatment on the expression of β-arrestin-2 in the lung tissue in sepsis-induced acute lung injury in mice.Methods Thirty female Ktmming mice,aged 6 weeks,weighing 18-20 g,were randomly divided into 3 groups(n =10 each):sham operation group(group S); sepsis group(group CLP)and penehyclidine hydrochloric pretreatment group(group PHC).Sepsis was induced by cecal ligation and puncture(CLP)in groups CLP and PHC.Penehyclidine hydrochloric 0.45 mg/kg was injected intraperitoneally at 1 h before CLP in group PHC.While the equal volume of normal saline was given instead of penehyclidine hydrochloric in groups S and CLP.At 12 h of CLP,the animals were sacrificed,and the lung tissues were removed for determination of MPO activity(by colorimetry),IL-6 content(by ELISA),β-arrestin-2 mRNA and protein expression(by RT-PCR and Western blot respectively).Blood samples and bronchoalveolar lavage fluid were collected to calculate pulmonary vascular permeability index(PV PI).Results Compared with group S,PVPI,IL-6 content and MPO activity were significantly increased,the expression of β-arrcstin-2 protein was significantly down-regulaled while the expression of β-arrestin-2 mRNA was up-regulated in group CLP,and PVPI,IL-6 content and MPO activity were significantly incrcased,the expression of β-arrestin-2 protein was significantly up-regulated,while the expression of β-arrestin-2 mRNA was down-regulated in group PHC(P ＜ 0.05).Compared with group CLP,PVPI,IL-6 content,and MPO activity were significantly decreased,the expression of β-arrestin-2 protein was significantly up-regulated,while the expression of β-arrestin-2 mRNA was dow n-regulated in group PHC(P ＜ 0.05).Conclusion PHC pretreatment can attenuate the lung injury induced by sepsis in mice through up-regulating the expression of β-arrestin-2 protein.
