Effect of PM2.5 on sympathetic innervation by stimulating the secretion of cardiac nerve growth factor in healthy rat hearts
10.3760/cma.j.issn.1008-1372.2012.06.001
- VernacularTitle:PM2.5对大鼠心脏交感神经分布的影响及其与心肌神经生长因子表达的关系
- Author:
Jun DUAN
;
Luhong CONG
;
Gang LI
;
Li YI
;
Yuannan KE
;
Yifeng ZHOU
- Publication Type:Journal Article
- Keywords:
Particulate matter/adverse effects;
Cardiovascular diseases/etiology;
Heart/innervation;
Sympathetic nervous system/pathology;
Nerve growth factor/metabolism
- From:
Journal of Chinese Physician
2012;14(6):721-724
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo assess the effect of PM2.5 on sympathetic innervation and the relationship with cardiac nerve growth factor in healthy rat hearts,identify the ability to the sympathetic nerve reconstruction,and explore the possible arrhythogenic mechanism of PM2.5.MethodsForty healthy SD rats were instilled into trachea with two different solutions twice per week for four weeks:control group with saline and experimental group with PM2.5 25 mg/kg.Then these rats were killed,and biatrial appendages,two ventricular anterior walls were left.Immune cytochemical staining of cardiac nerves was performed using anti-tyrosine hydroxylase antibodies and cardiac nerve growth factor was detected by western blotting.ResuitsCompared with the control group,both the density of sympathetic nerves and the expression of cardiac NGF protein in the experimental group were much higher in the left ventricular anterior wall ( P＜0.01 and P＜0.05) and in the right ventricular anterior wall ( P＜0.01 and P＜0.05).ConclusionsPM2.5 can induce regional sympathetic hyperinnervation in both ventricular anterior walls in healthy rats by stimulating the NGF protein secretion,and the sympathetic nerve reconstruction effect of PM2.5 was confirmed.
