Effects of intestinal ischemia-reperfusion on cerebral microgllal activation in rats
10.3760/cma.j.issn.0254-1416.2012.05.011
- VernacularTitle:肠缺血再灌注对大鼠脑组织小胶质细胞活化的影响
- Author:
Jun ZHOU
;
Kexuan LU
;
Jicheng WEI
;
Xiaobin WANG
;
Xianling TANG
;
Wenqi HUANG
- Publication Type:Journal Article
- Keywords:
Intestines;
Reperfusion injury;
Microglia;
Brain
- From:
Chinese Journal of Anesthesiology
2012;32(5):559-562
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of intestinal ischemia-reperfusion(I/R)on cerebral microglial activation in rats.Methods One hundred and twenty-eight healthy male SD rats weighing 250-300 g were randomly allocated to one of two groups(n =64 each):group sham operation(group S)and intestinal I/R group.Intestinal I/R was produced by occlusion of superior mesenteric stery for 90 main followed by reperfusion.Sixteen animals were sacrificed at each of the 4 time points:2,6,24 and 48 h of reperfusion in each group.Their intestines were obtained for microscopic examination.Their brains were harvested for detection of microglial activation (by immuno-histochemistry).The reactive oxygen species(ROS),MDA and NO contents and SOD,nitric oxide synthase(NOS)and inducible nitric oxide synthase(iNOS)activities in the brain were measured.Results The microglia were in quiescent condition.Ibal staining was negative or light in group S.Intestinal I/R significantly increased intestinal Chiu score,cerebral microglial activation at 6,24 and 48 h of repeffusion which peaked at 24 h of reperfusion in group I/R as compared with group S.Cerebral ROS,MDA,NO levels and NOS,iNOS activities were significantly higher while SOD activity was significantly lower in group I/R than in group S.Concluslon Intestinal I/R can activate microglia and induce the release of nitrogen and oxygen free radicals resulting in cerebral injury.
