Mechanism of dexamethasone inhibiting U937 cell adhesion and phagocytose function

VernacularTitle:地塞米松抑制U937细胞黏附与吞噬功能的作用机制
Author: Dong LIU ; Xingyun CHEN ; Renping XIONG ; Ping LI ; Yalei NING ; Yan PENG ; Yan ZHAO ; Nan YANG ; Yuanguo ZHOU
Publication Type:Journal Article
Keywords: Dexamethasone; Phagocytose; Non-genomic effect
From: Chinese Journal of Trauma 2012;28(5):466-469
CountryChina
Language:Chinese
Abstract: ObjectiveTo investigate the mechanism of dexamethasone (Dex) in inhibiting monocyte adhesion and phagocytose function.Methods Under the stimulation of phorbo1-12-myristate-13-acetate (PMA),U937 monocytes cultured in vitro were treated with Dex and Fasudil respectively.The adhesion rate of U937 monocles to human umbilical vein endothelial cells (HUVECs) and their phagocytic ability of India ink were studied.The protein content and activity of rho-associated coiled-coil protein kinase 1 ( ROCK1 ) as well as the effects of mifepristone and cycloheximide on Dex were determined.ResultsBoth DEX and Fasudil could significantly inhibit the adhesion tate and phagocytosis of U937 cells stimulated by PMA and suppressed the activity of ROCK1.While mifepristone and cycloheximide could not alter these effects of DEX.ConclusionDEX interferes with the adhesion and phagocytosis function of U937 cells by inhibiting ROCKI activity.