Effect of matrix metalloproteinase 9 in inflammatory response of coronary artery bypass grafting and correlation analysis of matrix metalloproteinase 9 and tumor necrosis factor alpha,interleukin 8
10.3760/cma.j.issn.1673-4904.2012.17.011
- VernacularTitle:基质金属蛋白酶9在冠状动脉旁路移植术炎性反应中的作用及与肿瘤坏死因子α、白细胞介素8的相关性分析
- Author:
Gang MA
- Publication Type:Journal Article
- Keywords:
Coronary artery bypass,off-pump;
Matrix metalloproteinase-9;
Tumor necrosis factor-alpha;
Interleukin-8
- From:
Chinese Journal of Postgraduates of Medicine
2012;35(17):31-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of the serum matrix metalloproteinase 9(MMP-9) in coronary artery bypass grafting(CABG) under cardiopulmonary bypass (CPB) and the correlations between the serum MMP-9 and tumor necrosis factor alpha (TNF-α),interleukin 8 (IL-8).Methods Twenty-two patients who subjected to CABG were selected.Artery blood samples were collected at the following time points:before intubation (T0),15 min after initiation of CPB (T1),the end of operation (T2),2 h after operation (T3) and 6 h after operation(T4).The serum MMP-9,TNF-α and IL-8 levels were detected by ELISA and analyzed.Results The serum MMP-9 levels at T1,T2,T3 and T4 were (102.82 ± 19.03),( 428.80 ± 32.91 ),( 305.64 ± 29.75 ),( 157.78 ± 22.18 ) μg/L,respectively,which were significantly higher than that at To [ (38.67 ± 10.15 ) μ g/L] (P ＜ 0.05 ).The serum MMP-9 level gradually increased during CPB and reached the peak at T2,and then decreased.The serum TNF- α and IL-8 levels had the same change as MMP-9,the peak levels at T2 were (287.06 ±24.58),(143.12 ± 16.36) ng/L,respectively.The serum MMP-9 level had positive correlation with TNF- α and IL-8 levels ( r =0.69,0.54,P ＜ 0.05 ).Conclusions CPB can lead to the increased release of MMP-9,TNF-α and IL-8.TNF-α and IL-8 may induce the activation and release of MMP-9.The serum MMP-9 can contribute to the general inflammatory reaction of CPB.
