The relationship between the change of aquaporin4,brain edema and neuronal function score of fluid percussion brain injury in rats
10.3760/cma.j.issn.1674-6554.2012.05.003
- VernacularTitle:实验性大鼠液压冲击脑损伤神经功能评分与脑水肿、水通道蛋白4表达的关系
- Author:
Guozhu SUN
;
Zongmao ZHAO
;
Leifang YANC
;
Xinggang LIU
;
Mugang WANG
;
Yangjun HAN
- Publication Type:Journal Article
- Keywords:
Brain injury;
Fluid percussion;
Brain edema;
Aquaporin4
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2012;21(5):392-394
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the relationship between neuronal function score,brain edema and aquaporin4(AQP4) expression of fluid percussion brain injury in rats.MethodsThe fluid percussion models of brain injury of rats were established by using the improved device.Nervous function score,brain water content,histological changes,AQP4 expression were observed by Shapira and Wahld method,dry-wet measure,light microscopy,immunohistochemistry and western blot at 1 h,6 h,12 h,24 h,3 d and 7 d after operation respectively.ResultsNervous function score in TBI group decreased at 12 h( 11.17 ± 1.32),reached its minimum at 24 h( 10.17± 0.75),and recoved partially at 3rd day( 10.66 ± 1.37 ).The water content obviusly increased in those of TBI group at 12h( (80.27 ±1.47)％ ),reached its peak at 24h( (82.19 ±0.97)％ ),and then began to drop at 3d ( (8 1.74 ± 1.69 ) ％ ),while Western blot showed that AQP4 immunoreactive expression obviusly increased at 12 h (OD:0.65 ±0.05),reached its maximum at 24h( OD:0.72 ±0.08),and decreased at 3d( OD:0.56 ±0.07),and immunohistochemistry showed the same trendency of AQP4 expression as that of Western blot.The linear regression analysis indicated that nervous function score had a negtive correlation with expression of AQP4 in edematous brain and change of brain water content respectively ( r =- 0.615,P ＜ 0.01 ; r =- 0.605,P ＜ 0.05 ).ConclusionNervous function score of fluid percussion brain injury in rats decrease,has negative relationship with brain edema and AQP4 expression,and possible mechanisms is that AQP4 is indirectly involed in nerve function impairment by mediating brain edema.
