Effect of propofol on spontaneous transient outward K+ currents in mouse cerebral arterial smooth muscle cells
10.3760/cma.j.issn.0254-1416.2012.03.009
- VernacularTitle:异丙酚对小鼠脑动脉血管平滑肌细胞自发性瞬时外向钾电流的影响
- Author:
Xueru LIU
;
Xiaoqiu TAN
;
Yan YANG
;
Xiaorong ZENG
;
Xianling TANG
- Publication Type:Journal Article
- Keywords:
Propofol;
Potassium channels,calcium-activated;
Muscle,smooth,vascular;
Cerebral arteries
- From:
Chinese Journal of Anesthesiology
2012;32(3):298-300
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of propofol on the spontaneous transient outward K+ currents in mouse cerebral arterial smooth muscle cells.Methods Kunming mice of both sexes,weighing 18-22 g,were used in this study.Vascular smooth muscle cells were freshly isolated from cerebral arteries in two steps.Five cells were chosen and studied.When holding potential was - 30 mV,spontaneous transient outward K+ currents were recorded before and after the application of 56 μmol/L propofol by perforated whole-cell patch-clamp technique.The amplitude,frequency,area under the curve and half time width of spontaneous transient outward K+ currents were analyzed.Results Propofol 56 μmol/L significantly increased the amplitude,frequency and area under the curve of spontaneous transient outward K+ currents.There was no significant change in the half time width of spontaneous transient outward K+ currents after administration,Conclusion Propofol can activate spontaneous transient outward K+ currents in mouse cerebral arterial smooth muscle cells,and thus induces vascular smooth muscle relaxation.
