Neurotoxicity of intrathecal doxepin in rats
10.3760/cma.j.issn.0254-1416.2012.03.013
- VernacularTitle:鞘内注射不同浓度多塞平对大鼠的神经毒性
- Author:
Zhifei XU
;
Kechang HUANG
- Publication Type:Journal Article
- Keywords:
Doxepin;
Injections,spinal;
Drug toxicity
- From:
Chinese Journal of Anesthesiology
2012;32(3):311-313
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the neurotoxicity of intrathecal (IT) doxepin in rats.Methods Twenty-four adult male Wistar rats weighing 250-300 g in which intrathecal micro-catheter was successfully implanted without complications were randomly divided into 4 groups ( n =6 each):control group ( group N ) and 3 doxepin groups receiving IT doxepin 40,20 and 10 mmol/L 0.2 μl/g respectively (groups D40,D20,D10 ).In N group normal saline was injected IT instead of doxepin.The animals were killed at 6 h after IT administration.The lumbar segment of the spinal cord was removed for microscopic examination and determination of myelin basic protein (MBP) content in the spinal cord using a double-antibody sandwich (ELISA).Results The severity of neuronal damage and decrease in MBP content in the spinal cord induced by IT doxepin were dose-dependent.Conclusion The neurotoxicity induced by IT doxepin is dose-dependent.
