2-ME-induced apoptosis of K562 cells and its mechanism

VernacularTitle:二甲氧雌二醇诱导K562细胞凋亡作用及其凋亡机制
Author: Suwei ZHANG ; Qiaoxin ZHANG ; Caixia WANG ; Jinghua LIN ; Yingmu CAI
Publication Type:Journal Article
Keywords: Apoptosis; Molecular Mechanisms of Action
From: Chinese Journal of Primary Medicine and Pharmacy 2012;19(9):1288-1290
CountryChina
Language:Chinese
Abstract: Objective To study the effect of 2-methoxyestradiol (2-ME) on proliferation,apoptosis and its mechanism.Methods K562 cells were treated with 2-ME of different concentrations and time in vitro.Cell apoptosis rate was measured by flow cytometry(FCM).Activity of NF-Kappa B in nucleus was detected by electrophoretic molility shift assay(EMSA).Results After treatment with 2-ME,K562 cells apoptotic rate increased significantly.After treatment with 4μmol/L 2-ME for 24h、36h、48h,the activity of Caspase-3 and Caspase-9 were significantly higher and activity of NF-Kappa B in nucleus was significantly lower.Conclusion The present study showed that 2-ME induced apoptosis of K562 cells via active Caspase-3 and Caspase-9 and inhibit the activity of NF-kappa B in nucleus.This study provided useful experimental data for clinical application of 2-ME.