Synergy effects of octreotide combined with cisplatin and 5-fluorouracil on inhibition of lung adenocarcinoma cell line A549
10.3760/cma.j.issn.1673-422X.2012.06.021
- VernacularTitle:奥曲肽联合顺铂和5-氟尿嘧啶对人肺腺癌细胞株A549抑制的增效作用
- Author:
Jinkun PAN
;
Baoping LI
;
Lei ZHANG
;
Hongwei REN
;
Feng XU
- Publication Type:Journal Article
- Keywords:
Carcinoma,non-small cell lung;
Octreotide;
Drug synergy;
Isobologram
- From:
Journal of International Oncology
2012;39(6):469-472
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether octreotide,as somatostatin analogue,can enhance the sensitivity of the human lung adenocarcinoma cell line A549 to chemotherapeutic drugs.Methods Different concentration of octretide,cisplatin and 5-Fluorouracil (5-Fu) was respectively acted on the lung adenocarcinoma cell line A549.The absorbance value was tested by colorimetry through MTT method to evaluate the effect of octreotide,cisplatin,5-Fu or the three drugs combined respectively after 48 hours.Each drug concentration had six holes and it repeated three times.The effects of combination therapy was analysed with isobologram.Results It was proved that octreotide could inhibit the proliferation of A549 cells in a dose-dependent manner at the concentration range of 1.3 mg/L ～ 166.7 mg/L.The inhibition rate was dose-dependent which was higher when octreotide combined with cisplatin and 5-Fu than it alone.It has statistically significant difference (P ＜ 0.05 ).The effect plots of IC50 were located in the synergy areas of isobologram.Conclusion It can be concluded that octreotide could inhibit the proliferation of A549 cells in vitro.This inhibition enhances when octreotide is combined with cisplatin and 5-Fu.Octreotide can enhance the susceptibility of A549 cells to cisplatin and 5-Fu.
