Differentiation and spermiogenesis of in vitro human spermatocytes

VernacularTitle:人类生精细胞在培养过程中的分化与变形
Author: Huaixiu WANG ; Hong LI ; Caixia YUAN ; Yingli CAO ; Qin QIN ; Chongzhi BAI
Publication Type:Journal Article
Keywords: Spermatocytes; Flow cytometry; Meiosis; Cell culture techniques
From: Chinese Journal of Urology 2012;33(6):455-458
CountryChina
Language:Chinese
Abstract: Objective To investigate the possibility of differentiation and spermiogenesis of spermatocytes under in vitro condition. Methods Testis biopsy was done in 11 cases with non-obstructive azoospermia.Cells were prepared from 9 samples with spermatocytes and cultured in medium containing follicle stimulating hormone 50 U/L and testosterone 1 μmol/L.Sperms and cells of other types were counted and the proportion of every cell type was calculated before and 24 hours after culture.Flow cytometry was conducted before and 24 hours after culture in 2 cases to analyze the ploidy of the cells. Results The proportion of sperm in 9 samples was ( 17.7 ± 8.9 ) ％ before culture and ( 25.6 ± 10.3 ) ％ after culture ( P =0.004).Sperm increased significantly after culture.Flow cytometry demonstrated that the diagram of 4 n,2 n and 1 n converted to 2 n and broader 1 n. Conclusion Meiosis of spermatocytes and the transformation of spermatid into sperm could arise in in vitro culture.