Study on effect of 17-β estradiol on cultured vaginal wall fibroblasts from patient with pelvic floor dysfunction after stretch
10.3760/cma.j.issn.1008-1372.2012.05.010
- VernacularTitle:17-β雌二醇对受力后盆底功能障碍性疾病患者阴道壁成纤维细胞的生物学影响
- Author:
Pingren CHEN
;
Bin SHI
;
Yabing WANG
;
Dongxiao LI
;
Haijiang JIN
- Publication Type:Journal Article
- Keywords:
Estradiol/pharmacology;
Pelvic floor/physiopathology;
Vagina/physiopathology;
Fibroblasts/physiopathology
- From:
Journal of Chinese Physician
2012;14(5):610-614
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of 17-β estradiol on Collagen Ⅰ,lysyl oxidase like 1 (LOXL1),fibrinectin( FN )after stretch in the pelvic function dysfunction(PFD) patients vaginal wall fibroblasts cultured in vitro,and the effect in PFD pathogenesis.Methods Twelve patients with pelvic function dysfunction who under surgery in the second Hospital,Hebei Medical University from October 2009 to September 2010 were collected vaginal wall organization in this study.Use of tissue and collagenase digestion method for primary culture in the vaginal wall fibroblasts.After stretch with different concentrations of 17-β estradiol cultured fibroblasts.Total mRNA was extracted from fibroblasts and gene expression of Col Ⅰ,LOXL1,FN were measured.Results After stretch,the expression of Col Ⅰ ( 1.1872 ± 0.0733 vs 1.5035 ±0.0733,t =-4.815,P ＜0.01),LOXLI(0.7724 ±0.1873 vs 1.0855 ±0.0805,t =-5.111,P ＜0.01 ) were significant higher than before( P ＜0.01 ),the expression of FN (0.4290 ±0.1168 vs 0.4215±0.0830) was not significantly different ( P ＞0.05).The expression of all factor Col [ (3.0809 ±0.1862),LOXLI ( 1.5863 ±0.3241 ),FN( 1.1418 ± 1.0030) in high drug concentrations of 17-β estradiol were increased significant compared with controlled groups( P ＜ 0.01 ).Conclusions 17-β estradiol increased synthesis of extracellular matrix components from cultured vaginal wall fibroblasts of patient with pelvic floor dysfunction after stretch.Stretch and estrogen may play an important role in PFD pathogenesis.
