Effect of neuropeptide substance P on airway resistance in rats with asthma
10.3760/cma.j.issn.1673-4912.2012.03.018
- VernacularTitle:神经肽P物质对哮喘大鼠气道阻力的影响
- Author:
Miao LI
;
Yunxiao SHANG
;
Na LI
- Publication Type:Journal Article
- Keywords:
Neuropeptide substance P;
Asthma;
Airway resistance;
Rat
- From:
Chinese Pediatric Emergency Medicine
2012;19(3):276-278
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of neuropeptide substance P on airway resiatance in rats with asthma.MethodsSixty Wistar rats were randomly divided into 3 groups including normal control group(n =20),asthma group(n =20) and substance P group(n =20).Asthmatic rat model was established.We detected inspiratory resistance and expiratory resistance in the airway of rats in each group with the intervention of different concentrations (0.062 5,0.125 0,0.250 0,0.5000 mg/ml) of methacholine(MCh) by using animal pulmonary function analyzer.Substance P group rats were given substance P ( 10-7 mol/L,0.2 ml) when airway resistance was detected.ResultsWith the increase in MCh concentration,the increase in airway resistance in rats was found,rat airway inspiratory resistance and expiratory resistance were all positively correlated with the concentration of MCh ( r=0.947,P＜0.05 ; r=0.965,P＜0.05 ).With the intervention of different concentration of Mch (0.0625,0.125 0,0.2500,0.5000 mg/ml),the significant differences were found among the three groups in rat airway inspiratory resistance ( F=73.4,89.3,91.2,106.4,P＜0.05) and expiratory resistance(F=67.3,77.5,83.4,126.5,P＜0.05).Airway resistance to MCh concentration response curves were shifted upward significantly in asthma group compared with normal control group and in substance P group compared with asthma group.Inspiratory resistance and expiratory resistancc in asthma group were significantly increased compared with normal control group( P＜0.05)and in sutstance P group compared with asthma group( P＜0.05 ).ConclusionSubstance P can increase asthmatic rat's airway resistance and the airway resistance increase with the increasing of MCh concentration.
