Serum proteomic analysis of patient with severe acute pancreatitis before and after treatment
10.3760/cma.j.issn.1674-1935.2012.03.001
- VernacularTitle:重症急性胰腺炎患者治疗前后血清蛋白组学的变化
- Author:
Jun REN
;
Kaihuan YU
;
Peng MA
;
Jia YU
;
Chen CHEN
;
Aimin ZHANG
- Publication Type:Journal Article
- Keywords:
Pancreatitis,acute necrotizing;
Proteome;
Serum;
Forecasting
- From:
Chinese Journal of Pancreatology
2012;12(3):147-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the different expression of the serum proteome of patient with severe acute pancreatitis (SAP) before and after treatment,and search for the protein that can predict SAP improvement.Methods SELDI-TOF-MS was used to analyze the expression of serum protein spectrum of 20 SAP patients before treatment and 7 days after treatment,and make the APACHE Ⅱ score at the same time.Results The APACHE Ⅱ score was 10.70 ± 3.47 in patients with SAP 7 days after treatment,which were significantly lower than that before treatment ( 13.00 ± 2.21,P ＜0.05 ).One hundred thirty-two spectral peak clusters were detected before and after treatment,there were 27 proteins that had significantly different signal intensities had been detected from 2000 Da to 50000 Da (P ＜ 0.05 ).The peak cluster at 47 000 Dawas significantly down-regulated after SAP attenuation.While the peak clusters at 1l700 Da and 15900 Dawere significantly up-regulated after treatment.Conclusions Serum analysis with SELDI-TOF MS can detect the different expression of the SAP serum proteomics before and after treatment,the increased expression of the protein with molecular weight of 11500 Da and 15900 Da and the decreased expression of 4700 Da may be asignal of improvement of SAP.
