Relationship between TLR4 expression and angiogenesis in human pancreatic adenocarcinoma
10.3760/cma.j.issn.1674-1935.2012.03.008
- VernacularTitle:Toll样受体4在胰腺癌组织中的表达及其与肿瘤血管生成的关系
- Author:
Xiaohua MAN
;
Yunliang SUN
;
Yanfang GONG
;
Hongyu WU
;
Zhaoshen LI
- Publication Type:Journal Article
- Keywords:
Toll-like receptor 4;
Pancreatic neoplasms;
Angiogenesis,pathologic;
Immunohistochemistry
- From:
Chinese Journal of Pancreatology
2012;12(3):167-169
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate Toll-like receptor-4 (TLR4) protein expression in human pancreatic adenocarcinoma,and to evaluate the relationship between TLR4 protein expression and angiogenesis.Methods Sixty-two surgically resected human pancreatic adenocarcinoma specimens and 35 normal para-cancerous tissues were investigated for TLR4 protein expression by immunohistochemical SP methods,and CD31 antibody was used to mark microvascular endothelial cells and determine the microvessel density (MVD).The correlation among TLR4 protein expression and MVD and clinicopathologic features of pancreatic adenocarcinoma were analyzed.Results TLR4 protein positive expression rate and MVD in human pancreatic adenocarcinoma was 74.2％ (46/62) and 47.3 ± 13.5,respectively,which were significantly higher than those in the normal pancreatic tissue [17.1％ (6/35),12.6 ±4.8; P ＜0.01].TLR4 protein positive expression rate in the cases with lymph node metastasis was 83.8％,which was significantly higher than that in the cases without lymph node metastasis (60.0％,P =0.036).TLR4 protein positive expression rate in the patients with stage Ⅲ and Ⅳ of TNM classification was 85.3％,which was significantly higher than that in the patients with stage Ⅰ and Ⅱ (60.7％,P=0.028).MVD was closely related to tumor size,lymph node metastasis and TNM stage of pancreatic adenocarcinoma (P =0.008,0.036,0.010).There was a strong positive correlation between TLR4 protein expression and MVD (r =0.534,P ＜0.01 ).Conclusions TLR4 protein expression is closely related to the development and progression of human pancreatic adenocarcinoma and its potential mechanism is related to the promotion of tumor angiogenesis.
