Mild hyperthermia affects chemoresistance of gemcitabine in pancreatic cancer PANC1 cell line
10.3760/cma.j.issn.1674-1935.2012.03.014
- VernacularTitle:温和高温下胰腺癌细胞株PANC1对吉西他滨耐药性的影响
- Author:
Minghao LIU
;
Shude LI
;
Zhaoshen LI
;
Jun GAO
;
Hongyu WU
;
Yanfang GONG
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Mild hyperthermia;
Gemcitabine;
Drug therapy
- From:
Chinese Journal of Pancreatology
2012;12(3):189-191
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of mild hyperthermia on chemoresistance of gemcitabine in the pancreatic cancer cell line PANC1.Methods The PANC1 cell was treated with gemcitabine ( 1 μmol/L) for 1 h,then was heated at 37℃,42℃ and 45℃ for 1 hour,and was cultured for 0 h,24 h,48 h.Cell growth was analyzed by CCK-8 assay.Cell apoptosis was analyzed by Annexin Vfluorescein isothiocyanate ( FITC)/propidium iodide (PI) staining.Results The proliferation of cells at 42℃ and 45℃ for 24 h and 48 h were significantly lower than that at 37℃ (0.96 ± 0.05,0.88 ± 0.03vs 1.05 ±0.02;1.28 ±0.04,0.94 ±0.04vs 1.49 ±0.09;t =4.367,25.120,P ＜0.05).The proliferation of cells at 45℃ was significantly lower than that at 42℃ (t =3.348,11.732,P ＜0.05).The cell apoptosis rates at 37℃,42℃,45℃ after 48 h were (7.125 ±0.064)％,(9.985 ±0.615)％,(14.845 ± 1.987)％,the difference among the 3 groups was statistically significant (t =10.320,9.832,4.575,P ＜0.05).Conclusions Mild hyperthermia can reduce chemoresistance of gencitabine in pancreatic cancer cell PANC1.
