Regulatory effect of tripterygium wilfordii polyglycoside on expression of epidermal growth factor receptor family in collagen induced arthritis rats
10.3760/cma.j.issn.1007-7480.2012.03.010
- VernacularTitle:雷公藤多苷对胶原诱导性关节炎大鼠表皮生长因子受体家族表达的影响
- Author:
Yi JIANG
;
Shenghao TU
;
Yukun XIA
;
Zhe CHEN
;
Dong CHANG
;
Hongwei YANG
;
Yonghong HU
- Publication Type:Journal Article
- Keywords:
Models,animal;
Arthritis,rheumatoid;
Receptor,epidermal growth factor;
Gene,ErbB-2;
Tripterygium wilfordiipolyglycoside
- From:
Chinese Journal of Rheumatology
2012;16(3):187-190
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the regulatory effect of Tripterygium wilfprdii polyglycoside (TWP) on the expression of EGFR and ErbB-2 induced arthritis rats.The effect of TWP on arthritis was also explored.MethodsAfter the model of CIA rats were established,the expression of EGFR and ErbB-2 in the synovium and articular cartilage were tested by immunohistochemical stain and real time PCR.ANOVA was used for statistical analysis.ResultsThe protein and mRNA expression of EGFR and ErbB-2 in the synovium (EGFR 0.268±0.059,ErbB-2 0.25±0.04,EGFR mRNA:14.2±0.55,ErbB-2 mRNA 23.46±3.64) and articular cartilage (EGFR 0.193±0.018,ErbB-2 0.217±0.033,EGFR mRNA:4.16±0.50,ErbB-2 mRNA 9.23±0.66) of the model group were significantly higher than those of the control group(P＜0.01).After being treated with TWP and MTX,the protein and mRNA expression of the EGFR and ErbB-2 decreased markedly (P＜0.01).Conclusion EGFR and ErbB-2 may play an important role in the pathogenesis of arthritis development.The molecular mechanism that TWP can treat synovitis and bone destruction of RA is related to the inhibition of EGFR and ErbB-2.
