Study on qualitative alteration in renal tubular epithelial cell induced by high glucose
10.3760/cma.j.issn.1008-6315.2012.06.022
- VernacularTitle:高糖诱导肾小管上皮细胞转化的作用
- Author:
Junhui LI
- Publication Type:Journal Article
- Keywords:
Renal tubular epithelial cell;
Transforming growth factor-β1;
Vimentin;
Smad 2
- From:
Clinical Medicine of China
2012;28(6):622-624
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of transforming growth factor-β1 (TGF-β1)and Smad anchor for receptor activation(SARA) in high glucose induced epithelium to mensenchymal transition(EMT) in proximal tubule HKC cells.Methods The model of EMT in HKC cells by high glucose treatment was developed.After stimulation by 25 mmol/L glucose for 0,12,24,and 48 h,the cells were collected and total protein was extracted.Vimentin,E-cadherin,TGF-β1,SARA and Smad2 expression were detected by western blot analysis.Results At 0,12,24,and 48 h after treated with 25 mmol/L glucose the expression of E-cadherin was 1.13 ±0.31,0.74 ±0.14,0.63 ±0.18 and 0.32 ±0.12 respectively,with significantly difference between each group ( Ps ＜ 0.05 ) ; the expression of vimentin in HKC cells increased from 0.23 ± 0.09,0.34 ± 0.04,0.83 ±0.15 to 1.02 ±0.22 signifiantly(Ps ＜0.05).High glucose treatment also upregulated the protein expression of TGF-β1,with expression of 0.25 ± 0.08,0.44 ± 0.12,0.72 ± 0.21,and 0.92 ± 0.28 after treated with 25 mmol/L glucose for 0,12,24,and 48 h ( Ps ＜ 0.05 ).The protein expression of SARA significanlty decreased from 0.83 ±0.21,0.54 ±0.13,0.42 ±0.09 to 0.35 ±0.12(Ps ＜0.05),and the protein expression of Smad2 also significantly decreased from 1.14 ± 0.22,0.82 ± 0.15,0.61 ± 0.17 to 0.30 ± 0.13 ( Ps ＜ 0.05 ).Conclusion The upregulation of TGF-β1 might play a key role in high glucose induced EMT in proximal tubule HKC cells.The downregulation of SARA and Smad2 protein might contribute to TGF-β1 mediated renal fibrosis.
