Study on the activity of liposome doxorubicin for reversing multidurg resistance of tumor cells and its mechanism
10.3760/cma.j.issn.1008-6706.2012.10.019
- VernacularTitle:脂质体阿霉素逆转肿瘤多药耐药的活性和机制研究
- Author:
Lixia XUE
;
Lihua JIN
- Publication Type:Journal Article
- Keywords:
Liposome doxorubicin;
Multidrug resistance;
Glycoprotein;
Vincristine;
Verapamil
- From:
Chinese Journal of Primary Medicine and Pharmacy
2012;19(10):1476-1477
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the multidrug resistance(MDR) reversal activity of a novel compound liposome doxorubicin(PLD) and its mechanism.MethodsMTT assay was used to evaluate MDR reversal activity of PLD in P-gp expressing tumor cell lines,KBv200 and MCF-7/ADR.ResultsPLD had a strong reversal in vivo activity,recognized the strong reversal activity than verapamil reversal activity,in 5.0μmol/L concentration of multi-drug resistant cell KBv200 increased sensitivity to vincristine of 45 times.KBv200 PLD-dependent increased in intracellular concentration of rhodamine accumulation(0,2.5,5.0,10μmol/L).Mainly to its cardiac toxicity,bone marrow suppression and hair loss and other side effects were significantly reduced.ConclusionPLD was an efficient modulator,mainly through the continuous accumulation to tumor tissue,tumor local drug concentration,enhanced anti-tumor activity,
