Study on Sudan Black B-loaded poly (lactide-co-glycolide) (SB-PLGA) contrast agents for tumor lymph node imaging
10.3760/cma.j.issn.1004-4477.2012.05.026
- VernacularTitle:载苏丹黑高分子超声造影剂增强兔淋巴结显影的实验研究
- Author:
Chengcheng NIU
;
Yuanyi ZHENG
;
Zhigang WANG
;
Haitao RAN
;
Yang SUN
;
Weixiang SONG
;
Yufang YOU
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Lymph nods
- From:
Chinese Journal of Ultrasonography
2012;21(5):446-448
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare polyclactic-co-glycolic acid(PLGA) polymer ultrasound contrast agent loading sudan black dye (SB-PLGA) and study the enhancement effect of it on rabbit lymph nodes imaging and as a sentinel lymph node (SLN) biopsy in the feasibility and value of the indicator.Methods SB-PLGA ultrasound contrast agent was made by double emulsion and freeze-drying methods.Thigh subcutaneous rabbit VX2 tumor model of eight lymph nodes were set up with the foot pad subcutaneous injection of contrast agents to get contrast-enhanced ultrasound imaging of the popliteal lymph node and the second inguinal lymph node stations.Then the blue stained lymph nodes were resected by popliteal lymph node dissection and the second station lymph nodes were also observed after 30 mins respectively,lymph nodes were examined by frozen sections HE staining in parallel.Results SB-PLGA microbubbles had a tight size distribution.Ultrasound imaging can be significantly enhanced lymph node imaging,the second station lymph node imaging was not obvious.Popliteal lymph node dissection for lymph node staining,30minutes later,frozen sections HE staining were seen within the lymph node contrast agent present in the lymphatic sinus and a large entry of macrophages.Inguinal lymph node dissection showed the second station of lymph nodes no blue staining.Conclusions SB-PLGA ultrasound contrast agent is good for sentinel lymph node imaging and biopsy indicator.Contrast agent retention and accumulation in the lymph nodes by the uptake of macrophages may be associated with the mechanisms of lymph node enhanced imaging.
