The B lymphocytes D8/17 antigen expression and characteristics of patients with rheumatic heart disease in Guangdong
10.3760/cma.j.issn.0578-1426.2012.05.007
- VernacularTitle:D8/17抗原在风湿性心脏病患者中的表达及特性
- Author:
Jianguang CHEN
;
Taiming DONG
;
Zhihong CHEN
;
Rong LI
;
Jian SU
;
Hongsui WU
- Publication Type:Journal Article
- Keywords:
Rheumatic heart disease;
Biological markers;
D8/17 antigen
- From:
Chinese Journal of Internal Medicine
2012;51(5):353-356
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the D8/17 antigen expression of patients with rheumatic heart disease (RHD) in Guangdong province and study the antigen's characteristics.Methods The level of D8/17 antigen expression on B lymphocytes was determined with flow cytometry assay in 96 RHD patients and 83 unaffected controls.The percentage of B-cells expressing the D8/17 antigen having more than 10％ was considered to be positive. D8/17 antigen was extracted by immunopreeipitation,and the antigen characteristics was analyzed by tandem mass spectrometry. Results The mean percentage of B-cells expressing the D8/17 antigen was (85.36 ± 15.15)％ in the RHD patients and (82.89 ±4.55)％ in the controls,with no significant difference between the two groups ( P =0.436).Moreover,the positive rate of the D8/17 cxprcssion was 100％ in either the RHD patients or the controls.The molecular weight of D8/17 antigen was found to be 40 000-67 000,and the purified protein was most likely to match moesin or β-actin.ConclusionsB-cell antigen D8/17 is not associated with RHD in Guangdong province of China.Moesin or β-actin is the most likely protein to match D8/17 antigen.
