Establishment and evaluation of rabbit unstable atherosclerotic plaque model induced by cold stress
10.3760/cma.j.issn.0254-9026.2012.04.019
- VernacularTitle:冷应激建立家兔动脉粥样硬化不稳定斑块模型的方法和评价
- Author:
Xiaohui ZHENG
;
Xue GAO
;
Yan LI
;
Liwen LIU
;
Xiaomin YI
;
Lei CHEN
;
Yongfeng ZHAO
;
Hongping SONG
;
Rongqing ZHANG
;
Haichang WANG
- Publication Type:Journal Article
- Keywords:
Hypothermia;
Stress;
Celiac artery;
Arteriosclerosis;
Neovascularization,pathologic
- From:
Chinese Journal of Geriatrics
2012;31(4):330-334
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and evaluate unstable atherosclerotic plaque model in abdominal aorta induced by cold stress. Methods Sixty male New Zealand white rabbits were randomly divided into three groups:cold stress group fed with high fat diet and followed by balloon induced arterial wall injury of abdominal aorta at week 2 and exposed to cold (4℃) for 1 h per day except for the first postoperative week,balloon-injury group treated by high fat diet plus balloon-injury, control group fed a normal chow without any treatment. Pathological changes of atherosclerotic plaques among these groups were evaluated at 20 weeks. Meanwhile, serum concentrations of blood lipid,oxidized low density protein(ox-LDL),hypersensitive C-reaction protein (hs-CRP)and interleukin (IL)-8 were determined. Results There was no difference in blood lipid level between cold-stress and balloon-injury groups.Serum concentrations of ox-LDL[(56.1 +14.3)mg/L vs.(42.9± 13.8)mg/L],hs-CRP[(149.1+78.3)mg/L vs. (94.5±57.3)mg/L],IL-8 [(97.6±17.9)μg/L vs.(57.5±18.3)μg/L]and macrophage infiltration[(30.9±5.6)％ vs,(18.7±4.8) ％] were significantly higher in cold stress group than in balloon-injury group (t =2.78,6.91,14.94,6.88,all P＜0.05). Higher angiogenesis rate of atherosclerotic plaque in cold-stress group (23/31,74.1％) was observed in comparison with group balloon-injury(5/25,20,0％)(x2=16.26,P＜0.05). Conclusions Establishment of rabbit unstable atherosclerotic plaque model induced by cold stress in synergy with high fat diet and balloon-injury is feasible, which is superior to conventional method through high fat diet plus balloon-injury surgery.
