Study on the diferences of gene expression of peripheral blood mononuclear cells between ACCPA+ and ACCPA patients with rheumatoid arthritis
10.3760/cma.j.issn.1007-7480.2012.05.013
- VernacularTitle:抗环瓜氨酸肽抗体阳性和阴性类风湿关节炎患者外周血单个核细胞基因表达谱差异的研究
- Author:
Xide LIU
;
Xutao HONG
;
Long CAI
;
Yunqing WANG
;
Fang WANG
;
Yingying FENG
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Gene expression profiles;
Anti-cyclic citrullinated peptide antibody;
Microarray
- From:
Chinese Journal of Rheumatology
2012;16(5):338-342
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the differences of gene expression of peripheral blood mononuclear cell (PBMC) between the anti-cyclic citrullinated peptide antibody (ACCPA) positive and ACCPA negative patients with rheumatoid arthritis (RA) by microarray analysis.Methods Total RNA was extracted from PBMC of 5 ACCPA positive and ACCPA negative patients with RA,and age-and sex-matched control subjects respectively.Ⅲumina oligonucleotide microarray was used to characterize 47231gene expression profile for each sample.Results Among the target genes,88 differentially expressed genes were identified in RA patients.Fifty-one up-regulated genes and 37 down-regulated genes were found in RA patients compared to those in control subjects (fold change＞1.5).The differential expression of genes were associated with apoptosis,cytokine,signal identification protein,chemotaxis factor etc.There were 20 differential expression genes between the ACCPA positive and ACCPA negative patients with RA,9 up-regulated genes and 11 downregulated genes were found.The differential expression genes were associated with protein biding,,translation control,signal identification protein,cell cycle,metabolism etc.Conclusion There are differential gene expression between the ACCPA positive and ACCPA negative patients with RA.Genes screened from the target such as IFI,KIR,CHI3L1 can provi-de important information for further study and treatment of RA.
