Role of Akt/GSK-3β signaling pathway in neuropathic pain in mice
10.3760/cma.j.issn.0254-1416.2012.02.014
- VernacularTitle:Akt/GSK-3β信号转导通路在小鼠神经病理性痛中的作用
- Author:
Jinhua BO
;
Xiaofeng SUN
;
Juan ZHANG
;
Wei ZHANG
;
Zhengliang MA
;
Xiaoping GU
- Publication Type:Journal Article
- Keywords:
Protein-serine-yhreonine kinases;
Glycogen synthase kinase 3;
Neuralgia
- From:
Chinese Journal of Anesthesiology
2012;32(2):184-186
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the role of Akt/glycogen synthase kinase-3β (GSK-3β) signaling pathway in neuropathic pain in mice.MethodsThirty-six male C57BL/6 mice,aged 7-8 months,weighing 20-25 g,were randomly divided into 2 groups:sham operation group (group.S,n =9) and chronic constrictive injury (CCI) group (n =27).CCI was produced by placing loosely constrictive ligatures around the common sciatic nerve.Six animals were taken from each group and paw withdrawal threshold to mechanical stimulation (PWMT)and paw withdrawal latency to thermal stimuli (PWTL) were measured on day 1 before operation and on day 1,3,5,7,10,14,17 and 21 after CCI.Three mice were sacrificed on day 3 after CCI in group S and on day 1,3,5,7,10,14 and 21 after CCI in group CCI.The L3-5 segment of the spinal cord was removed for determination of the expression of Akt,phospho-Akt and phospho-GSK-3β by Western blot.Results Compared with group S,PWMT was significantly decreased and PWTL was significantly shortened on day 1-21 after CCI,the expression of Akt was significantly up-regulated on day 7-21 after CCI,and the expression of phospho-Akt and phospho-GSK-3βwas significantly up-regulated on day 1-21 after CCI in group CCI ( P ＜ 0.05).ConclusionAkt/GSK-3β signaling pathway is involved in the development and maintenance of neuropathic pain in mice.
