Effect of propofol on IL-1β and TNF-α release from BV-2 microglia cells induced by lipopolysaccharide and the role of Toll-like receptor 4
10.3760/cma.j.issn.0254-1416.2012.02.018
- VernacularTitle:异丙酚对LPS诱导BV-2小胶质细胞IL-1β和TNF-α释放的影响及TLR4在其中的作用
- Author:
Mingyan SU
;
Bo GUI
;
Yanning QIAN
- Publication Type:Journal Article
- Keywords:
Propofol;
Lipopolysaccharides;
Interleukin- 1 beta;
Tumor necrosis factor-alpha;
Microglia;
Toll-Like receptor 4
- From:
Chinese Journal of Anesthesiology
2012;32(2):198-200
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of propofol on IL-1β and TNF-α release from BV-2 microglia cells induced by lipopolysaccharide (LPS) and the role of Toll-like receptor 4 (TLR4).MethodsBV-2 microglia cells were seeded in 96-well plates and randomly divided into 4 groups ( n =12 each):control group,LPS group,propofol group (group P) and LPS + propofol group.In group LPS,the cells were incubated with LPS 1 μg/ml for 24 h.In group P,the cells were incubated with propofol 30 μmol/L for 24 h.In group LPS + propofol,the cells were incubated with LPS 1 μg/ml and propofol 30 μmol/L for 24 h.The concentrations of TNF-α ( at 6 h of incubation) and IL-1β (at 24 h of incubation) in the supernatant were detected by ELISA.TLR4 mRNA expression was detectedat at 6 h of incubation by RT-PCR.TLR4 protein expression was detected at 24 h of incubation by Western blot.ResultsCompared with control group,IL-1β and TNF-α release was significantly increased,and the expression of TLR4 mRNA and protein up-regulated in groups LPS and LPS + propofol ( P ＜ 0.05).Compared with group LPS,IL-1β and TNF-α release was significantly decreased,and the expression of TLR4 mRNA and protein down-regulated in group LPS + propofol (P ＜ 0.05 ).Conclusion Propofol can inhibit IL-1β and TNF-α release from BV-2 microglia cells induced by LPS and inhibition of TLR4 expression may be involved in the mechanism.
