Role of ATP sensitive potassium channel in protective effects of post-conditioning with hydrogen sulfide against hepatic ischemia-reperfusion injury in rats
10.3760/cma.j.issn.0254-1416.2012.02.027
- VernacularTitle:ATP敏感性钾通道在硫化氢减轻大鼠肝缺血再灌注损伤中的作用
- Author:
Jin DU
;
Qian WANG
;
Jun ZHANG
;
Guolin WANG
- Publication Type:Journal Article
- Keywords:
KATP channels;
Hydrogen sulfide;
Reperfusion injury;
Livers
- From:
Chinese Journal of Anesthesiology
2012;32(2):228-231
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the role of ATP sensitive potassium (KATP ) channel in protective effects of post-conditioning with hydrogen sulfide against hepatic ischemia-reperfusion (I/R) injury in rats.Methods Thirty adult male SD rats weighing 220-250 g were randomly divided into 5 groups ( n =6 each):sham operation gorup (group S) ; hepatic I/R group; NaHS group; glibenclamide + NaHS group (group G) and 5-hydroxydecanoate (5-HD) + NaHS group (group 5-HD).Hepatic ischemia was produced by 60 min occlusion of left and middle lobe blood flow (about 70％ of the whole liver blood flow) followed by 6 h reperfusion in groups I/R,NaHS,G and 5-HD.NaH S 28 μmol/kg was administered intraperitoneally (IP) at 5 min before reperfusion in groups NaHS,G and 5-HD.Glibenclamide 6 mg/kg and 5-HD 10 mg/kg (KATP channel blockers) were administered IP at 5 min before IP NaHS in groups G and 5-HD respectively.Blood samples were collected at the end of 6 h of reperfusion for measurement of ALT and AST activities and liver specimens were obtained for microscopic examination and measurement of TNF-α content and MPO activity.ResultsLiver I/R significantly increased ALT,AST and MPO activities and TNF-α concentration in group I/R as compared with group S.NaHS administered at 5 min before reperfusion significantly attenuated hepatic I/R injury.Glibenclamide and 5-HD administered before NaHS could block the protective effect of NaHS against hepatic I/R injury.ConclusionHydrogen sulfide postconditioning can protect the liver from I/R injury by activating KATP channel.
