Metabolic features of acute necrotizing pancreatitis and chronic pancreatitis
10.3760/cma.j.issn.1674-1935.2012.01.016
- VernacularTitle:大鼠急性坏死性胰腺炎和慢性胰腺炎代谢特征分析
- Author:
Chunshu PAN
;
Chao MA
;
Bing TIAN
;
Jian WANG
;
Genjin YANG
;
Jianping LU
- Publication Type:Journal Article
- Keywords:
Pancreatitis,acute necrotizing;
Chronic pancreatitis;
Magic angle spinning nuclear magnetic resonance;
Principal components analysis
- From:
Chinese Journal of Pancreatology
2012;12(1):52-54
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the metabolite features of acute necrotizing pancreatitis (ANP) and chronic pancreatitis (CP) in rats.MethodsA total of 22 Wistar rats were divided into ANP group (n =7 ),CP group (n =6) and the control group (n =9).ANP model was induced peritoneous injection of 20％ Larginine,and the rats were sacrificed 12 hours later.CP model was induced by intravenously injection of DBTC (8 mg/kg body weight),and the rats were sacrificed after 2 months.The rats in the control group received same amount of saline.Serum amylase was determined and pancreatic tissues were pathologically examined.Metabolic changes of pancreatic tissues in vitro were studied by high resolution magic angle spinning nuclear magnetic resonance (MAS NMR ),and analyzed by using principal components analysis (PCA).Characteristic metabolites of ANP and CP were compared. Results Compared with the control group,increased leucine,iso-leucine and valine levels were observed in ANP group,however,the opposite trends were observed in CP group.Phosphocholine,glycerophosphocholine,choline levels were increased and fatty acids,lactate,betaine,glycine levels were decreased in both ANP and CP groups.The lipid content in CP group were significantly higher than that in ANP group and the increased taurine was only observed in CP group. Conclusions There were obvious metabolic features in pancreatic tissue in rats with pancreatitis disorders,and the increased taurine could be used as biomarker to discriminate ANP and CP.
