Effects of pyruvate sodium on energy metabolism of erythrocytes in dog blood during circulation in cardiopulmonary bypass machine
10.3760/cma.i.issn.0254-1416.2012.02.004
- VernacularTitle:丙酮酸钠对犬血在体外循环机内循环时红细胞能量代谢的影响
- Author:
Daming GOU
;
Hongjing TAN
;
Fangqiang ZHOU
;
Huijun CAI
;
Fubing LIU
- Publication Type:Journal Article
- Keywords:
Pyruvates;
Erythrocytes;
Energy metbolism;
Extracorporeal circulation
- From:
Chinese Journal of Anesthesiology
2012;32(2):152-153
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investgate the effects of pyruvate sodium on energy metabolism of erythrocytes in dog blood during circulation in cardiopulmonary bypass machine.MethodsTen healthy adult mongrel dogs of both sexes weighing 12-18 kg were anesthetized with intraperitoneal 2.5％ pentobarbital 25 mg/kg.Femoral artery was cannulated and 500 ml of blood was collected from femoral artery in each dog.Blood samples from the 10 dogs were divided into 2 equal parts (250 ml each).Each part was mixed with 250 ml lactated Ringer's solution (group C) or 250 ml pyruvate sodium (group P).Flow rate of 4 L/min was employed and blood temperature was kept at 30-32 ℃ during circulation in cardiopulmonary bypass machine.The blood sanples were collected before (baseline value,T1 ) and at 30,60,90,120 min of circulation (T2-5) for determination of ATP,ADP and AMP concentrations in RBC.ResultsThe ATP and AMP concentrations were significantly higher at T2-5,while ADP concentration was higher in group P than in group C.ConclusionPyruvate sodium can improve energy metabolism of erythrocytes in dog blood druing circulation in cardiopulmary bypass machine.
