Effects of sevoflurane on inhibition of invasive activity and migration of human lung adenocarcinoma cell line A549 by cisplatin
10.3760/cma.j.issn.0254-1416.2012.02.006
- VernacularTitle:七氟醚对顺铂抑制人肺腺癌细胞株A549侵袭和迁移能力的影响
- Author:
Hua LLANG
;
Miaoning GU
;
Chengxiang YANG
;
Xianjie WEN
;
Qiaoling ZHOU
- Publication Type:Journal Article
- Keywords:
Anesthetics,inhalation;
Cisplatin;
Neoplasm invasiveness;
Cell movement
- From:
Chinese Journal of Anesthesiology
2012;32(2):158-160
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of sevoflurane on inhibition of invasive activity and migration of human adenocarcinoma cell line A549 by cisplatin.MethodsThe.human lung adenocarcinoma cell line A549 was obtained from Shanghai Cell Biology Institute,Chinese Academy of Sciences and cultured in RPMI 1640 culture medium containing 10％ fetal calf serum.The cells were inoculated in culture plate and cultured for 24 h and randomly divided into 4 groups:control group; 2.5 ％ sevoflurane group ; cisplatin group and cisplatin + 2.5 ％sevoflurane group.In groups sevoflurane,cisplatin and cisplatin + sevoflurane the cells were exposed to 2.5％sevoflurane or/and cisplatin 10μmol/L for 4 h respectively.The invasive activity of the cells was evaluated by Transwell chamber assay.The migration of the cells was determined by wound healing assay.The expression of MMP-2,MMP-9,Ezrin,and Fascin in the cells was detected by Western blot.ResultsBoth 2.5％ sevoflurane and cisplatin depressed invasive activity and migration of the A549 cells and down-regulated MMP-2,MMP-9,Ezrin and Fascin expression in A549 cells.The inhibitory effects of cisplatin on the A549 cells were potentiated by 2.5 ％ sevoflurane.ConclusionSevoflurane can enhance the inhibition of invasive activity and migration of human adenocarcinoma cell line A549 by cisplatin.
