The application of the single cell fluid in detecting HER-2 gene or protein
10.3760/cma.j.issn.1008-6706.2012.06.003
- VernacularTitle:单细胞悬液在人表皮生长因子受体2检测中的应用
- Author:
Hongxun CHEN
;
Xingchang REN
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Epidermal growth factor;
Cytological techniques
- From:
Chinese Journal of Primary Medicine and Pharmacy
2012;19(6):806-807
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveUsing the single cell fluid to establish a kind of practical experimental method,to detect the HER-2 gene and protein of breast cancer,and to provide reliable basis for pathology diagnosis.MethodsThe single cell fluid of breast cancer was preparaed by Medimachine system.Sucking a little of the single cell fluid spreaded on the slide and to be examined by immunohistochemistry using antibodies to HER-2 and fluorescence in situ hybridization.ResultsThe cell membrane of breast cancer was positive for HER-2 by immunohistochemistry according to American society of clinical oncology of American pathologists guideline recommendations for HER-2 testing in breast cancer and the HER-2 gene of the single cell amplified obviously by fluorescence in situ hybridization which the ratio of the average of HER-2 and the average of CEP17 exceeded 2.2.ConclusionThe single cell fluid could be used as positive control when we detected the HER-2 gene or protein of breast cancer,which guaranteed the correction of the results of the sample to be detected.
