Isolation of intraperitoneal free cancer cells from colorectal cancer by immunomagnetic beads

VernacularTitle:免疫磁珠技术在结直肠癌腹腔内脱落癌细胞检测中的应用
Author: Honglei LIU ; Shunmao MA ; Ruifeng REN ; Yitao JIA ; Zhongxin LI
Publication Type:Journal Article
Keywords: Colorectal cancer; Immunomagnetic beads; Cytological techniques
From: Chinese Journal of Primary Medicine and Pharmacy 2012;19(6):804-805
CountryChina
Language:Chinese
Abstract: ObjectiveTo explore the value of immuomagnetic beads(IMB) technique for detection of intraperitoneal free cancer cells from colorectal cancer.MethodsPeritoneal lavage fluid was obtained from 80 patients with colorectal cancer during laparotomy.Peritoneal lavage cytology (PLC) and IMB were used to detect free cancer cells in peritoneal lavage fluid.10 patients with hysteromyoma during laparotomy were enrolled into the control group.ResultsThe positive rate of PLC was 8.8％ (7/80),the positive rate of IMB was 28.8％ (23/80).The positive case after useing PLC detect,IMB detect also was positive.The detected samples of control group were negative by these two methods.IMB was superior to PLC ( x2 =10.503,P =0.001 ).ConclusionIMB was more sensitive and specific than PLC,which could provide a effective method for finding intraperitoneal free cancer cells.