Application of quality control of immunohistochemical stainig in tissues slice
10.3760/cma.j.issn.1008-6706.2012.05.025
- VernacularTitle:免疫组化染色质量控制在组织切片中的应用
- Author:
Qin SHEN
- Publication Type:Journal Article
- Keywords:
Immunohistochemistry;
Staining;
Quality control;
Standardization;
Tissues slice
- From:
Chinese Journal of Primary Medicine and Pharmacy
2012;19(5):695-696
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the quality control and standardization of HE and immunohistochemical stainings in tissues slice.Methods In accordance with the standardization of quality control,HE and immunohistochemial stainings in tissues slice were performed,and the results were blindly compared before and after the standardization.Results The excellent rates of HE and immunohistochemical stainings in tissues slice after standardization were 92.3% and 91.1% respectively,and higher than those before standardization 78.9% and 74.9%(x2 =3.841,3.801,all P < 0.05); Diagnosis rate of 100 cases with liver biopsy tissue after standardization 86.0% was significantly higher than 73.0% before standardization(x2 =3.741,P < 0.05).Conclusion The quality of HE and immunohistochemical stainings in tissues slice enhanced after standardization.