Modified three-cuff technique for orthotopic intestinal transplantation in rats
10.3760/cma.j.issn.1673-4203.2011.12.004
- VernacularTitle:三套管法大鼠原位小肠移植动物模型的改进
- Author:
Ligong TANG
;
Lu YIN
;
Moubin LIN
;
Yonggang HE
;
Mingjun ZHANG
- Publication Type:Journal Article
- Keywords:
Rat;
Orthotopic;
Small bowel transplantation;
Cuff anastomosis;
Surgical microscope;
Animal model
- From:
International Journal of Surgery
2012;39(3):154-157
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo modify the technique of whole small bowel transplantation in rats to improve the survival.MethodsOne hundred and six SD rats served as donors and recipients to establish a homogeneous and orthotopic model of small bowel transplantation without fistula.Anastomosis of aorta and vein was done with three- cuff technique,the vena mesenterica superior of recipient was done under a surgical microscope.End-to-end anastomosis was performed between donor proximal small intestine and receptor jejunum,and also between the remote and receptor ascending colon.Surviving more than 3 days after surgery was defined as the operation was successful.ResultsThe operation was successfully carried out in 48 cases with the survival rate of 90.6％ (48/53).The average warm ischemic time was 0 minute,and the average cold ischemic time was(30 ±2.48)min.The survival rate( ＞7 d) was 97.9％ (47/48).ConclusionsOn the basis of three-cuff technique,the survival of SBT was greatly improved.When the vena mesenterica superior of recipient was done under a surgical microscope,the surgical training time was shortened.
