Effects of eritoran on the expressions of IL-1β, TNF-α and IFN-β mRNA in the basilar artery after subarachnoid hemorrhage in rabbits
10.3760/cma.j.issn.1673-4165.2012.03.007
- VernacularTitle:Eritoran对蛛网膜下腔出血兔基底动脉IL-1β、TNF-α和IFN-β mRNA表达的影响
- Author:
Dong WANG
;
Zhiyong YANG
;
Hua LU
;
Chao WANG
- Publication Type:Journal Article
- Keywords:
Subarachnoid Hemorrhage;
Eritoran;
Interleukin-1β;
Tumor Necrosis Factor-α;
Interferon-β;
Toll-Like Receptor 4;
Basilar Artery;
Disease Models,Animal;
Rabbits
- From:
International Journal of Cerebrovascular Diseases
2012;20(3):193-197
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of eritoran on the expressions of the inflammatory cytokines interleukin-1β (IL-1β),tumor necrosis factor-α (TNF-α) and interferon-[ (IFN-β) mRNA in the basilar artery after subarachnoid hemorrhage (SAH) in rabbits.Methods Atotal of 36 healthy adult male New Zealand white rabbits were randomly allocated into three groups:SAH (n =12),normal saline (n =12) and eritoran (n =12) groups.A SAH model was induced by injection of autologous arterial blood into cisterna magnatwice.An equal amount of cerebrospinal fluid was displaced with the saline in the normal saline group.An equal amount of autologous non-heparinized arterial blood was injected immediate after the replacementof cerebrospinal fluid in the SAH group.Eritoran 1.5 mg/kg was injected intravenously immediately after the blood injection via the cisterna magna each time in the eritoran group.The food intake and neurological deficit were assessed.The expressions of IL-1β,TNF-α and IFN-β mRNA in the basilar artery were detected by real-time fluorescence quantitative polymerase chain reaction.Results The food intake scores (1.20 ± 0.41 vs.2.20 ±0.61; t =53.073,P =0.002),the neurological deficit scores (1.46 ± 0.32 vs.2.6 ± 0.08; t =306.431,P =0.001),the expressions of IL-1β (1.22 ±0.48 vs.2.38 ±0.06,P =0.000),TNF-α (1.39 ±0.07 vs.3.32 ±0.21,P =0.000) and IFN-β (1.51 ±0.08 vs.2.18 ±0.05,P =0.000) in Eritoran group were all significantly lower than those in the SAH group.Conclusions Eritoran may downregnlate the expressions of inflammatory cytokines IL-1β,TNF-α and IFN-β mRNA in the basilar artery after SAH in rabbits,increasing food intake,and improving neurological deficits.
