Effect of gastrodin on rat vascular smooth muscle cell migration induced by platelet-derived growth factor-BB
10.3760/cma.j.issn.1673-4165.2012.03.006
- VernacularTitle:天麻素对血小板衍生生长因子-BB诱导大鼠血管平滑肌细胞迁移的影响
- Author:
Lihua ZHU
;
Hongjing GUAN
;
Lang WANG
;
Song TIAN
;
Da YANG
;
Mingyue FU
;
Hong JIANG
- Publication Type:Journal Article
- Keywords:
Gastrodin;
Cell Movement;
Muscle,Smooth,Vascular;
Rats
- From:
International Journal of Cerebrovascular Diseases
2012;20(3):189-192
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of gastrodin on rat vascular smooth muscle cell (VSMC) migration induced by platelet-derived growth factor-BB (PDGF-BB) and its possible mechanisms.Methods Enzyme digestion method wasused to obtain rataorticVSMCs and be purified bypassage.Immunofluorescence staining was used to identify VSMC marker proteins.A PDGF-BB induced cell migration model was established.Transwell chamber assay was used to evaluate the effect of gastrodin on PDGF-BB induced VSMC migration.Western blots were performed to detect the phosphohorylation levels of c-jun N-terminal kinase (JNK).Results The purity of primary cultured VSMC was more than 99％.The VSMC migrated number in the PDGF-BB group was 85.2 ± 3.486 per field.It was significantly more than 42.5 ± 1.927 per field in the control group (t =9.981,P＜0.001),and gastrodin was enable to make PDGF-BB induced the number of VSMC migration significantly reduce to 71.3 ± 1.783 per filed (t=3.550,P =0.002).Western blots analysis showed that gastrodin inhibited PDGF-BB induced JNK phosphorylation (0.190 ± 0.015 vs.0.190 ± 0.015; t =14.548,P =0.000).Conclusions Gastrodin inhibits PDGF-BB induced VSMC migration,its mechanisms may be associated with the inhibition of the JNK signaling pathway activation.
