Membrane molecular weight cut off impacts immunological safety of a novel bioartificial liver system
10.3760/cma.j.issn.1007-8118.2012.03.013
- VernacularTitle:膜截流分子量在新型生物人工肝免疫安全性中的作用研究
- Author:
Yue ZHANG
;
Xiaolei SHI
;
Bing HAN
;
Jinyang GU
;
Xuehui CHU
;
Jiangqiang XIAO
;
Haozhen REN
;
Jiaojun TAN
;
Yitao DING
- Publication Type:Journal Article
- Keywords:
Bioartificial liver;
Membrane molecular weight cut off;
Porcine hepatocyte;
Bone marrow mesenchymal stem cells
- From:
Chinese Journal of Hepatobiliary Surgery
2012;18(3):200-204
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of membrane molecular weight cut off in our bioartificial liver(BAL)system.Methods Beagle dogs were used for a model of acute liver failure through D-galactosamine administration.The acute liver failure Beagles were divided into two groups by the membrane molecular weight cut off.Group A was treated with BAL containing 200 kDa retention rating membrane.Group B was treated with BAL containing 1200 kDa retention rating membrane.Each group underwent two six-hour BAL treatments that were performed on day 1 and day 21.BAL medium were examined and levels of IgG,IgM,and complement hemolytic unit of 50％(CH50)antibodies were measured in all Beagles and.Results BAL treatment was associated with a significant decline in levels of CH50.1200 kDa group experienced a significant increase in levels of IgG and IgM after two BAL treatments.Significant levels of canine proteins were detected in BAL medium from 1200 kDa group.Conclusions Xenogeneic immune response in the BAL system was influenced by membrane molecular weight cut off.
