Effect of genistein on cell cycle and apoptosis in PC12 cells transfected with APP695MT gene
10.3760/cma.j.issn.1674-6554.2012.02.009
- VernacularTitle:三羟基异黄酮对转染APP695基因PC12细胞周期和凋亡的影响
- Author:
Fengbin WANG
;
Zhenyu DING
;
Youhua KONG
;
Yanan WANG
;
Ning LI
- Publication Type:Journal Article
- Keywords:
Genistein;
APP695;
PC12 cells;
Cell cycle;
Apoptosis
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2012;21(2):123-125
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of Genistein (GST) on cell cycle and apoptosis in PC12 cells transfected App695MT gene.MethodsPC12 cells were transfected with pIRES2-EGFP plasmid or pIRES2-EGFP/APP695MT expression plasmid,and then were divided into control vectortransfected group,APP695 transfected group and GST treatment group.Flow cytometry was applied to detect cell cycle and apoptosis,laser confocal microscope was used to observe morphological changes of cell apoptosis.ResultsCompared with control vectortransfected group,PC12 cells in APP695 transfected group increased significantly in G0 and G1 phase,and less into S phase,cell proliferation index was decreased significantly( (55.6 ±0.57)％,P＜0.0l ),apoptosis rate was increased significantly( (77.10 ± 12.53)％,P＜0.01 ).Emitted red fluorescence increased significantly when cell death,cell body swelling,organelle disintegration,nuclear condensation or fragmentation.Compared with APP695 transfected group,PC12 cells in GST( 15μ mol/L) treatment group,decreased significantly in G0 and G1 phase,and more into S phase,cell proliferation index was increased significantly ( ( 61.57 ± 0.47 ) ％,P ＜ 0.01 ),apoptosis rate was decreased significantly ( (46.00 ± 8.43 ) ％,P ＜ 0.01 ).Cell death was significantly reduced red fluorescence,emitted green fluorescence was significantly enhanced,compared with APP695 transfected group cell morphology improved.ConclusionGST can improve APP695MT gene caused cell cycle arrest,promote cell to S phase transition,reduce apoptosis rate in PC12 cells,and have a protective effect on transfected cells.
