δ-opioid receptor activation by protein kinase C pathway inhibits acid-induced liver cell apoptosis
10.3760/cma.j.issn.1007-8118.2012.03.015
- VernacularTitle:δ阿片受体激活通过蛋白激酶C途径抑制人肝细胞凋亡
- Author:
Bo TANG
;
Yi ZHANG
;
Rui LIANG
;
Jidong SUI
;
Xueli JIN
;
Peng YUAN
;
Liming WANG
- Publication Type:Journal Article
- Keywords:
Opioid receptor;
Liver cells;
Apoptosis;
Caspase-3;
Protein kinase C
- From:
Chinese Journal of Hepatobiliary Surgery
2012;18(3):211-214
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effects of δ-opioid receptor activation in serumdeprivation induced apoptosis of human liver cells and the proposed protein kinase C(PKC)pathway mechanism.Methods MTT assay was used to detect the survival rate of human liver cells in vitro and Annexin V-FITC/PI double staining was used to detect the cell apoptosis rate.Flow cytometry was used to analyze cell cycle,RT PCR used to analyze the PKC mRNA and Western Blot analysis was used for detecting the protein expression of PKC and Caspase-3.Results After serum-deprivation for 48h of cultured human liver cells in vitro,significant liver cell apoptosis occurred.The apoptosis was suppressed by δ-opioid receptor activation,which manifested as a slower rate of apoptosis,decreased expression of Caspase-3and increased expression of PKC.After GF109203X was added,the inhibitory effects of DADLE decreased markedly.Conclusion Activation of δ-opioid receptor on the membrane of human liver cells has inhibitory effects on serum-deprivation induced apoptosis of liver cells.The underlying mechanism may be associated with PKC pathway activation.
