Effects of recombinant lentivirus encoding human apM1 gene on platelet-derived growth factor inducedmesangial cell proliferation
10.3760/cma.j.issn.1000-6699.2012.03.016
- VernacularTitle:人脂联素基因重组慢病毒对血小板源性生长因子诱导的肾小球系膜细胞增殖的影响
- Author:
Yanxin SU
;
Huacong DENG
;
Jian LONG
;
Mingxiang ZHANG
;
Zhougui PENG
- Publication Type:Journal Article
- Keywords:
Mesangial cell;
Lentivirus;
Adiponectin;
AMP-activated protein kinase
- From:
Chinese Journal of Endocrinology and Metabolism
2012;28(3):226-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of recombinant lentivirus encoding human apM1 gene ( LentiapM1-EGFP) on platelet-derived growth factor (PDGF) induced human mesangial cell (HMC) proliferation and intracellular AMP-activated protein kinase(AMPK) signaling pathway.Methods Protein expression of apM1 in cell culture supernatant of HMC transfected with Lenti-apM1-EGFP was detected by ELISA.The effect of human adiponectin on cell proliferation and cell cycle was assessed by [ 3 H ] thymidine incorporation assay and Flow cytometry respectively.The phosphorylation of AMPK was detected by Western blotting.Results Lenti-apM1-EGFP had no significant toxicity on HMC.The 50 multiplicity of infection (MOI) of the Lenti-apM1-EGFP efficiently infected HMC,and made it stable expression of adiponectin protein ( 149.6 ± 12.8 ) μg/L.PDGF-induced HMCs proliferation was significantly inhibited by adiponectin.When co-treatment with compound C,an AMPK inhibitor,the inhibitory effort was reversed.The phosphorylation level of AMPK was increased in HMC transfected Lenti-apM1-EGFP compared to that of control.Conclusions Adiponectin antagonizes stimulatory effect of platelet-derived growth factor on mesangial cell proliferation by AMPK signaling.
