Histone acetylation in peripheral blood mononuclear cells of type 2 diabetic patients

VernacularTitle:2型糖尿病患者外周血单个核细胞组蛋白乙酰化研究
Author: Can HOU ; Ming ZHAO ; Xia LI ; Yijun LI ; Yi LIN ; Qianjing LU ; Zhiguang ZHOU
Publication Type:Journal Article
Keywords: Diabetes mellitus,type 2; Peripheral biood mononuclear cells; Histone acetylation
From: Chinese Journal of Endocrinology and Metabolism 2012;28(3):183-185
CountryChina
Language:Chinese
Abstract: Objective To study histone acetylation patterns in peripheral boiood mononuclear cells (PBMC) from type 2 diabetic patients PBMC form 12type 2 diabetic patients and 12 healthy contrcl subiects were collecyed The global histone H3/H4 acetylation was determined by assay kit The differetial expression of the histone acetyltransferases and histone deacetylases were measured by real time PCR.Results increased H3/H4 acetylation was observed in PBMC of type 2 diabetic patients compared with control subjects (H3:0.134±0.035vs0.181±0.032,p<0.05;H4:0.266±0.070VS0.324±0.062,P<0.01).the mRNA levels of p300,CREBBP,HDAC2,HDAC7,and STRTL in patients PBMC compared with healthy control subieects were 3.11±0.38,2.78±0.45,3.55±0.30,0.77±0.37 and 0.40±0.25 folds respectively conclusion histone acetylation appears abnormal in PBMC of type 2 diabetic patients.