Effects of 5-aza-2'-deoxycytidine and trichostatin A on the methylation and expression of TFPI-2 gene in human pancreatic cancer Panc-1 cell line
10.3760/cma.j.issn.1007-8118.2012.03.019
- VernacularTitle:5-氮杂-2'-脱氧胞苷和曲古菌素A对胰腺癌Panc-1细胞系TFPI-2基因甲基化水平及表达的影响
- Author:
Zhigang TANG
;
Hao ZHENG
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Gene expression;
Trichostatin A
- From:
Chinese Journal of Hepatobiliary Surgery
2012;18(3):226-229
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of 5-aza-2'-deoxycytidine(5-Aza-dC)and trichostatin A(TSA)on the methylation and expression of TFPI-2 gene in pancreatic cancer Panc-1 cells.Methods We treated pancreatic cancer Panc-1 cells with 5-Aza-dC and TSA alone or combined.TFPI 2 gene's DNA,mRNA and protein were determined by MSP,RT-PCR and Western blot.Results The hypermethylation of TFPI-2 gene in Panc-1 cells was reversed after treated with 5 Aza-dC alone or in combination with TSA.The re-introduction or raised expression of TFPI-2 mRNA and protein was detected in Panc-1 cells after treated with 5 Aza dC alone or in combination.However,we did not see the hypermethylation of TFPI 2 gene reversed nor detected TFPI 2 gene mRNA and protein after treated with TSA alone.Conclusions The methylation of the promoter region for the TFPI-2 gene is a main cause for its transcriptional activation in Panc-1 cells.The hypermethylation of TFPI-2 gene in Panc-1 cell was reversed and the expression of TFPI-2 gene mRNA and protein was detected after trea ted with 5-Aza-dC alone or in combination with TSA.Treatment of TSA alone does not have significant effect on TFPI-2 gene reexpression.
