Effect of alcohol exposure during pregnancy on learning and memory and H2S/CBS in the hippocampus of infant rats
10.3760/cma.j.issn.1674-6554.2012.02.011
- VernacularTitle:孕期饮用酒精对子代大鼠学习记忆及海马H2S/CBS系统的影响
- Author:
Chunyang XU
;
Ailin DU
;
Hongbo JIANG
;
Yan ZHANG
;
Wei HAO
;
Ruiling ZHANG
- Publication Type:Journal Article
- Keywords:
Alcohol exposure during pregnancy;
Infant rats;
Hydrogen sulfide
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2012;21(2):129-131
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of alcohol exposure during pregnancy on learning and memory and content of hydrogen sulfide(H2S) in the hippocampus of infant rats.MethodsThe animal models of alcohol exposure during pregnancy were made,and the learning and memory were evaluated by Y-maze in adult offspring.Content of H2S and activity of cystathionine-beta-synthase(GBS) in the hippocampus of the brain were evaluated with spectrophotometry;and CBS protein expression in the hippocampus was detected by immunohistochemistry.ResultsThe learning and memory ( (43.00 ± 15.33 ) times) of alcohol exposure during pregnancy group was significantly decreased compared with that of control and drinking groups (( 25.13 ± 12.35 )times and (26.12 ±11.95 ) times,P ＜ 0.05 ) ; spectrophotometry results showed that the content of H2S ( ( 30.32 ± 5.84 ) nmoL/g) of alcohol exposure during pregnancy group was significantly increased compared with that of control ( ( 52.51 ±7.85 ) nmol/g) and drinking groups( (49.93 ± 4.29 ) nmol/g),and the activity of CBS( ( 55.13 ± 4.45 ) nmol/g)of alcohol exposure during pregnancy group was significantly increased (P ＜ 0.01 ) compared with that of control ( (71.06 ± 5.58 ) nmol/g) and drinking groups( (69.96 ± 6.13 ) nmol/g) ; immunohistochemistry showed that the expressionof CBSproteinofalcoholexposureduringpregnancygroupwassignificantlyincreased.ConclusionThe damage effect of alcohol exposure during pregnancy on nerve system of infant rats may interrelate with down-regulation of H2S/CBS in the hippocampus.
