Apoptosis induced by HMME mediated photodynamic therapy in murine hepatoma cell line MM45T-Li
10.3760/cma.j.issn.1673-4181.2012.01.005
- VernacularTitle:HMME介导的光动力疗法诱导鼠肝癌MM45T-Li细胞凋亡的实验研究
- Author:
Yuxiao CUI
;
Yingxin LI
;
Hui MA
;
Liqing ZHENG
- Publication Type:Journal Article
- Keywords:
Photodynamic therapy;
Apoptosis;
Hepatocellular carcinoma;
MM45T-Li
- From:
International Journal of Biomedical Engineering
2012;35(1):18-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of HMME mediated photodynamic therapy (PDT) on apoptosis of murine hepatoma cell line MM45T-Li.MethodsThe photodynamic treatment on MM45T-Li cells was performed in vitro using HMME as photosensitizer and 630 nm laser as light source.The ceils were incubated with HMME of 2.5,5,10 and 20 μg/ml for 4 h respectively,and then irradiated with laser at different dosages.The dark toxicity of HMME and the activity of cells after PDT were evaluated by MTT assay.The effect of PDT on apoptosis were observed by Hoechst fluorescent staining analysis.ResultsMTT assay showed that there were no significant effects on cell growth in each concentration group of HMME when the cells were not exposed to light.The inhibition ratio of cells increased with laser dosage at HMME concentration of 10 and 20 μg/ml.Chromatin condensation,nuclear pyknosis and karyorrhexis were observed after PDT by Hoechst fluorescent staining analysis.The apoptosis rate increased in groups of 5.4 J/cm2 and 7.2 J/cm2 compared with that of control (P＜0.05).Conclusion HMME mediated PDT can efficiently induce the apoptosis of MM45T-Li cells.
