The influence of down-regulation of Tspan 8 by shRNA on metastasis and invasion of hepatocellular carcinomas
10.3760/cma.j.issn.1007-8118.2012.02.014
- VernacularTitle:shRNA下调Tspan8基因对肝癌细胞侵袭与转移的影响
- Author:
Jincai WU
;
Zhuori LI
;
Kailun ZHOU
;
Bin JI
;
Youfei QI
;
Zhensheng ZHANG
;
Jinfang ZHENG
;
Jiacheng CHEN
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinomas;
Invasion;
Metastasis;
RNA interference
- From:
Chinese Journal of Hepatobiliary Surgery
2012;18(2):126-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Tspan 8 on metastasis and invasion of human hepatocellular carcinomas(HCC).Methods RT-PCR and western blot were used to detect the expressions of Tspan 8 in HCC cell lines,HCC and matched nontumorous tissues.The expression of Tspan 8 was then down-regulated by LV/GFP/Tspan 8 in HCC cells.The expressions of Tspan 8 mRNA and protein were determined by RT-PCR and Western blot assay,respectively.The proliferation was examined by MTT,the expression of AMDM12 was assessed by Western blot,and the invasion ability of HCC cells was evaluated by transwells.Results A high level of Tspan 8 was found in high metastatic potential HCC cells,and the expression of Tspan 8 in HCC tissues was much higher than that in the matched nontumorous tissues. Down-regulation of Tspan 8 had no influence on the proliferation of HCC cells (P＞0.05),while it inhibited the expression of ADAM12 and the invasive ability of HCC cells (P＜0.01,P＜0.01 respectively).Conclusion Tspan 8 played an important role in invasion and metastasis of human hepatocellular carcinomas and down-regulation by LV/GFP/Tspan 8 inhibited the invasiveness of human hepatocellular carcinoma cells.
