Study of rapid endotoxin detection based on interdigital gold electrode and SAW
10.3760/ema.j.issn.1673-4181.2012.01.006
- VernacularTitle:基于叉指金电极和声表面波技术的细菌内毒素快速检测方法的研究
- Author:
Xiaohe YANG
;
Hao YANG
;
Xiaojun SHEN
;
Weimin LV
- Publication Type:Journal Article
- Keywords:
Endotoxin detection;
Interdigital gold electrode;
Surface acoustic wave;
Self-assembly
- From:
International Journal of Biomedical Engineering
2012;35(1):21-23
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish a method of rapid detection of bacteria endotoxin based on interdigital gold electrode and surface acoustic wave (SAW).MethodsWith the carriers of 3-APTES and glutaral pentanedial,Polymyxin B (PMB) was immobilized on interdigital gold electrode,and then reacted with the bacteria endotoxin' s biological active material (lipopolysacchride,LPS) selectively.Double differential circuit based on SAW was employed to complete the detection.Two output oscillation signals were differentiated through mixer and the endotoxin content was described by frequency shift.ResultsThe experimental results indicated that there was a liner relationship between the frequency shift and endotoxin content within the content range of 75-130 EU/ml.ConclusionComparing with traditional method,the detection time is shortened to 10 minutes,the sensitivity is 13.8 KHz/(EU·mL-1),and repeatability is above 90％.
